从2400种候选抗原中,结肠癌15种抗原被验证

肿瘤新抗原mRNA疫苗在加速审批的背景下,肿瘤突变抗原的研究价值越来越大,为此,我们通过文献挖掘,为读者整理了各种肿瘤的已验证肿瘤突变抗原清单,我们将陆续发布在GCBI知识库公众号、浩生医疗官网

我们从310篇文献中,整理出了2384种结肠癌中高表达的抗原肽。筛选出能激活T细胞活性的共有15种,详情如下:

抗原肽抗原蛋白呈递抗原的HLA验证文章
VNRWTRRQVILCETCLIVSSVKDSLPH domain leucine-rich repeat-containing protein phosphatase 1HLA class IImmunogenicity of somatic mutations in human gastrointestinal cancers. Science 2015
VKKPHRYRPGTVTLREIRRYQKSTEHistone H3.3HLA class IImmunogenicity of somatic mutations in human gastrointestinal cancers. Science 2015
RHRYLSHLPLTCKFSICELALQPPVRING finger protein 10HLA class IImmunogenicity of somatic mutations in human gastrointestinal cancers. Science 2015
PILKEIVEMLFSHGLVKVLFATETFHelicase SKI2WHLA class IImmunogenicity of somatic mutations in human gastrointestinal cancers. Science 2015
MTEYKLVVVGADGVGKSALTIQLIGTPase KRasHLA-C*08:02Immunogenicity of somatic mutations in human gastrointestinal cancers. Science 2015
LYCESVHNFProstatic acid phosphataseHLA-A*24:02Prostatic acid phosphatase as a target molecule in specific immunotherapy for patients with nonprostate adenocarcinoma. J Immunother 2005
KTLGIKYFSMTEVDRLGIGKArginase-1HLA class IArginase-1 targeting peptide vaccine in patients with metastatic solid tumors - A phase I trial. Front Immunol 2022
GDRFCLGQLSNAHRTSMAD4HLA-A*02:01T cell neoepitope discovery in colorectal cancer by high throughput profiling of somatic mutations in expressed genes. Gut 2017
GDRFCLGQLSNAHRTSMAD4HLA-A*02:01T cell neoepitope discovery in colorectal cancer by high throughput profiling of somatic mutations in expressed genes. Gut 2017
FVTQKRMEHFYLSFYTAEQLVYLSTE3 ubiquitin-protein ligase RNF213HLA class IImmunogenicity of somatic mutations in human gastrointestinal cancers. Science 2015
EESNLSFLKELLVRINRLDLLITYLCaspase-8HLA class IImmunogenicity of somatic mutations in human gastrointestinal cancers. Science 2015
DVDPGEHYILKTLGIKYFSMArginase-1HLA class IArginase-1 targeting peptide vaccine in patients with metastatic solid tumors - A phase I trial. Front Immunol 2022
DPDCVDRLLQCTQQAVPLFSKNVHSApoptosis inhibitor 5HLA class IImmunogenicity of somatic mutations in human gastrointestinal cancers. Science 2015
DLSIRELVHRILLVAASYSAVTRFIGamma-tubulin complex component 2HLA class IImmunogenicity of somatic mutations in human gastrointestinal cancers. Science 2015
CLGQLSNASMAD4HLA-A*02:01T cell neoepitope discovery in colorectal cancer by high throughput profiling of somatic mutations in expressed genes. Gut 2017

上述清单中抗原特异性T细胞的检测,需要抗原肽MHC荧光四聚体标记细胞,而后通过流式技术检测或分选细胞,我们可以提供定制生产 (定制咨询请扫描本页右侧联系人二维码)。

肿瘤突变抗原清单使用方法:

肿瘤微环境研究:

检测肿瘤浸润淋巴细胞中,肿瘤抗原特异性淋巴细胞的数量实现。具体方式是:根据清单内容,将抗原肽和HLA合成为抗原肽MHC复合物荧光四聚体,通过流式细胞检测方法,检测肿瘤浸润淋巴细胞的含量。

肿瘤免疫细胞的全身分布:

通过检测外周血中肿瘤抗原特异性淋巴细胞的数量实现。具体方法同上。

过继性肿瘤免疫细胞治疗:

使用清单中抗原肽,刺激淋巴细胞,而后使用抗原肽MHC复合物荧光四聚体或磁珠四聚体,通过流式分选或磁珠分选细胞,经体外细胞增殖后,回输体内,评估肿瘤抑制效果。

肿瘤抗原特异性TCR测序:

通过肿瘤浸润淋巴细胞、或外周血淋巴细胞,通过分选后进行单细胞TCR测序,获得抗原特异性T细胞的TCR序列,进一步进行TCR-T疗法研究。

肿瘤疫苗片段筛选:

综合上述过程,获得具有激活T细胞杀伤肿瘤细胞的经验证抗原肽MHC复合物,用于肿瘤疫苗构建。